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To further explore the restricted expression pattern of HSD2 and HSD1, we performed a simultaneous ribonuclease protection assay for both HSD1 and HSD2 transcripts in total RNA from a wide variety of tissues from male mice Figure 2. HSD1 was strongly expressed in total RNA from liver, kidney, stomach, lung, and fat. We also detected strong expression in total RNA from spleen and thymus, and weak expression in adrenal, small intestine, stomach, and epididymis. HSD2 was strongly expressed in kidney and colon as expected, with lower levels of expression in small intestine, stomach, and epididymis. Very low levels of expression were detected in total RNA from lung, thymus, and spleen. All other mouse tissues tested were negative for HSD2 at this level of sensitivity. No expression of either HSD1 and HSD2 was detected in bladder, testis, seminal vesicles, vas deferens, prostate, or skeletal muscle. Expression of HSD1 and HSD2 mRNA was detected by RNase protection in total RNA using 32Plabeled antisense probes for HSD1, a 270bp Xba I cDNA fragment encompassing exons 4 through 6, HSD2, and a 320 bp Sty IEcoRI fragment from exon 5. Total RNA (10 g) from various tissues was incubated with both probes (Probe) together overnight at 56 Yeast tRNA (tRNA) was incubated as a negative control, and size marker (M) was end labeled 1 kb ladder (GIBCO BRL).
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Product Info : GS7500 The GenShed shelter provides a convenient rigid weatherproof lockable place to store and operate a portable generator for backup power when connected to a transfer switch It provides outoftheway protection for your generator and frees up space in your garage for other itemsExhaust fumes and heat exit the GenShed shelter through the magnetic exhaust system while additional heat is dissipated through louvers mounted on the walls and rear of the Longchamp sacs enclosure The blower fan connects to a standard 120 volt receptacle on the generator to introduce fresh air http://www.projectlocus.org/Oakley-Sunglasses.html to the generator engine and only runs when the generator is running The high temperature sensor switch will shut off the generator in case of overheating Features Factory Installed Louvers PatentPending Magnetic Exhaust system PreInstalled Blower Fan High Temperature Sensor Switch Please Note ALL components included in the GenShed shelter kit MUST be installed in order for it to operate as designed and to avoid property damage or personal injury N Read more. Buy Online Buy Cheap Also Big Save Inexpensive Cheaper than Target Walmart BestBuy and Costco Online Fast Shipping GenTran GS7500 Generator Shelter for Portable Generators up to 7500Watt Big Save Prices. Deals Secure Order
As the main competitor of Luxottica, Safilo Group is the world's second largest eyewear company who also focuses on producing European eyeglass frames. Headquartered in Padova Italy, Safilo company now makes sunglasses and eyeglass frames. It business range is quite similar to Luxottica. Notable brands kept by Safilo include Safilo, Carrera, Oxydo and Smith Optics. The company also produces eyeglasses under licensed brands such as Dior, Gucci and Jimmy Choo and so on. In addition to these two great companies, there are still many other corporations in Europe developing European eyeglass frames under different brands. Cazal eyeglasses made in Germany were first introduced in the early 1980s and became a popular enjoyment by countless entertainers and athletes. European eyeglass frames are also available in some less well known names, like Giorgio Armani, Grotesque, Lafont, Mykita, Okki etc.
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